Download PDF by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N.: Applications of Chimeric Genes and Hybrid Proteins Part A:

By Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson

ISBN-10: 0121822273

ISBN-13: 9780121822279

The significantly acclaimed laboratory regular for greater than 40 years, Methods in Enzymology is among the so much hugely revered courses within the box of biochemistry. because 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless appropriate today--truly an important book for researchers in all fields of lifestyles sciences

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Additional resources for Applications of Chimeric Genes and Hybrid Proteins Part A: Gene Expression and Protein Purification

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2. 5 ml of 10 mM MgSO4. 3. A d d 50/zl of the cell suspension to each of five sterile test tubes. 4. Using a sterile Pasteur pipette, pick individual well-isolated plaques from the phage plate as agar plugs and transfer one, two, three, and four plugs, respectively, to each of the first four tubes. 5. Mix the phage and cells by vortexing the tubes briefly, making sure that the plugs are immersed in the cell suspension. Incubate at room temperature for 5 min. 6. A d d 2 ml of LB broth containing 10 mM MgSO4 to each of the five tubes.

Casadaban, J. Mol. Biol. 104, 541 (1976). CONSXRUCTIN6 lac VUSIONS IN E. coli [2] 27 lacA' lacY lacZ'trpA' A J l~l~'fJffffA A ~ S cI N ~r B RA 'trpA'lacZ Ac N cI R J lacY lacA'~ S trpA' lacZ lacY lacA' ~'Jfffff~\~ J "X AR cI N S \ Ac Y cI RA ( P x ~ x " trpA'lacZ lacY lacA'~ D placMu50 cA Px ~1~ J X E Px# X"trpA' lacZ lacY lacA' K/cfffff~N~\~N~l J AR cI N ~ S FIG. 2. Adapted from Bremer et al. 2 (A) Schematic diagram of AplacMu50. Details of the construction of this complex vector can be found in Bremer et al.

Kiino and T. J. Silhavy,Z Bacteriol. 158, 878 (1984). [3] T a g g i n g E x p o r t e d P r o t e i n s Alkaline Phosphatase By Using Escherichia Gene Fusions coli COLIN MANOIL Escherichia coli alkaline phosphatase (AP) requires export out of the reducing environment of the cytoplasm to form disulfide bonds and fold into an active form. 1 Hybrid proteins consisting of an alkaline phosphatase 1 A . D e r m a n a n d J. B e c k w i t h , J. Bacteriol.

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Applications of Chimeric Genes and Hybrid Proteins Part A: Gene Expression and Protein Purification by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson


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