By Dr. T. Powell (auth.), H. M. Piper, P. G. Spieckermann (eds.)
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Additional resources for Adult heart muscle cells: Isolation, properties and applications
Farmer BB, Harris RA , Jolly WW, Hathaway DR. Katzberg A, Watanabe AM , Withlow AL, Besch HR (1977): Isolation and characterization of adult rat heart cells. Arch Biochem Biophys 179: 545- 558 4. Gerards P, GrafW. Kammermeier H (1982): Glucose transfer studies in isolated cardiocytcs of adult rats. J Mol Cell Cardiol 14: 141-149 5. Halestrap AP (1976): Transport of pyruvate and lactate into human erythrocytes. Biochem J 156: 193 - 207 6. Kammermeier H. Kasenda S, Jung T, Lang U, GrafW (1979): Myocardial transcndothclial uptake of substrates and metabolites during dual tracer bolus administration.
Coleman Al, Paterson OS, Somerville AR (1979): The ~-adrenergic receptor of rat corpus luteum membranes. Biochem Pharmacol28: 1003- 1010 46 6. von Tscharner V and Bailey IA (1983): Non-invasive, kinetic measurements of )H-nitrendipinc binding to isolated rat myocytes by condensed phase radioluminescence. FEBS Lett 162: 185-188 7. von Tscharner V and Radda G K (1980): A st udy of changes in surface area and molecular interactions in phospholipid residues by condensed phase radioluminescence. Biochim Biophys Acta 60 I: 63 - 77 8.
E. first the type of kinetics ofL-lactate uptake, second its dependence on pyruvate and third its dependence on pH. The uptake kinetics with saturation and sigmoidal shape indicates carrier mediated uptake, however, not of simple substrate-carrier interaction determined by mass action ratio, since the latter should exhibit Michaelis-Menten-like kinetics. It rather points to a cooperative carrier system. When formally calculating according to Segel (13) a cooperative system with 4 binding sites would fit best to the data points.
Adult heart muscle cells: Isolation, properties and applications by Dr. T. Powell (auth.), H. M. Piper, P. G. Spieckermann (eds.)